Species Reactivity: Human Applications: Western Blot Shop ICAD Rabbit anti-Human, Polyclonal, Invitrogen at. Anti-ICAD (DFF) (cleaved form) Antibody from rabbit, purified by affinity chromatography Synonym: ICAD, DFF-45, DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit, Inhibitor of CAD, DNA degradation of chromosomal DNA, DNase find Sigma-Aldrich-ABC27 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich. There are recent reports that ICAD is a potential target for restoring a normal apoptotic signal transduction pathway in colon and brain cancer cells. ICAD Antibody, PA5-34818, from Invitrogen. In addition to its DNase inhibitory activity, ICAD acts as a CAD specific folding chaperone. IHC-plus DFFA / ICAD / DFF45 Antibody LS-B3186 is a pathologist validated IHC antibody Rabbit anti-Human DFFA / ICAD / DFF45. By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount. anti-rabbit peroxidase-conjugated secondary antibody the immunoreactive. The activation of the CAD/ICAD complex occurs through the caspase 3-mediated cleavage of ICAD at residues 117 and 224, which results in three ICAD fragments that are then released from CAD. There are no reviews for DFF45/ICAD Antibody (AF4679). primary DFFA (ICAD) antibody (LF-PA0058, Thermo Scientific, Rockford, IL. In non-apoptotic cells, CAD is complexed with its inhibitor, ICAD. ICAD is cleaved to be inactivated and allow caspase-activated DNase (CAD) to execute nuclear internucleosomal apoptotic DNA fragmentation. Although both ICAD-L and ICAD-S can bind and inhibit CAD, only ICAD-L was reported to be functional. Optimal working dilutions must be determined by end user. Key Applications: Western Blotting Application Notes: Western blot: 1:500-1:1000 Mouse lung tissue lysate can be used as a positive control and a 45 kDa band should be detected. ICAD has two isoforms: a functional isoform of 45kDa, ICAD-L/DNA fragmentation factor (DFF) 45 and a 35kDa isoform, ICAD-S/DFF35. 2-21 of mouse ICAD) is an antibody against ICAD/DFF for use in WB. ICAD is the inhibitor of caspase-3-activated DNase which is a substrate that controls nuclear apoptosis. ICAD antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ICAD.The immunogen is located within the last 50 amino acids of ICAD. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles. A hamster monoclonal antibody (mAb) against mouse CAD was established by immunizing.
#ICAD ANTIBODY DOWNLOAD#
For short-term storage (1-2 weeks), product can be stored at 4☌. Download scientific diagram No expression of CAD in ICAD-/- cells. Centrifuge briefly prior to opening the vial. Store product as a concentrated solution.
The PA5-34818 immunogen is recombit fragment corresponding to a region within amino acids 56 and 316 of ICAD (Uniprot IDNo.O00273). alpha polypeptide antibody DNA fragmentation factor, alpha subunit antibody DNAation factor 45 kDa subunit antibody H13 antibody ICAD antibody. These death signals finally cause the degradation of chromosomal DNA by activated DNase. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases.
DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis.PA5-34818 targets ICAD in WB applications and shows reactivity with Human samples. ICAD Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Antibodies should not be exposed to prolonged high temperatures. ICAD Polyclonal Antibody From Supplier Abbkine - Immunogen information: Synthesized peptide derived from the Internal region of human ICAD at AA range. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. For products with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.Īntibody can be stored at 4☌ for three months and at -20☌ for up to one year. Rabbit polyclonal ICAD antibody was raised against a peptide corresponding to amino acids 312 to 331 of mouse ICAD.Īntibody is supplied in PBS containing 0.02% sodium azide.ĭuring shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. Cell-based Manufacturing Media Platform.
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